Dengue Fever in Pakistan: Current Updates

Saba Irshad, Ayesha Saeed

Institute of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan

Corresponding Author’s email: saba.ibb@pu.edu.pk

Accept Date: Fab 08, 2012 01:47

Abstract :

Dengue virus (DNV) is the cause of dengue infections and Pakistan along with the world is now being overloaded with their patients. The molecular epidemiological studies reveal that there are four serotypes of the DNV circulating throughout the endemic countries. Non-structural glycoprotein (NS-1) causes the damage of hepatocytes along with the activation of the complement system which in turn causes the severity of the disease. The different modified forms of the PCR have been using for the detection and confirmation of the DNV which includes; Reverse Transcriptase Polymerase Chain Reaction (RT- PCR), Real-time PCR, Nested RT-PCR and Reverse transcription-loop-mediated isothermal amplification (RT-LAMP). Key Words: Dengue Shock syndrome, DHF, Fatal Disease