CHARACTERIZATION AND BIOCHEMICAL BEHAVIOR ASSESSMENT OF VACCINAL STRAIN OF PASTEURELLA MULTOCIDA TYPE 1 B6

Dildar Hussain Kalhoro1 , Rahmatullah Rind1 , Muhammad Saleem Kalhoro1 , Alam Zeb2 Ali Zaman2 , Shahnawaz Kumbhar 3 , Mazhar Hussain Mangi 1 and Rahimdad Birohi1

1Department of Veterinary Microbiology, Sindh Agriculture University Tandojam, Pakistan 3Department of Veterinary Pathology, Sindh Agriculture University Tandojam, Pakistan 2Gomal Collage of Veterinary Sciences, Gomal University D. I. Khan KPK, Pakistan

Abstract :

This study was carried out to record the morphological and biochemical properties of Pasteurella vaccinal strain. Pasteurella multocida Type 1 B-6 was observed as gram-negative, short rods, arranged singly, bipolar. Coccobacillary in shape and recorded as non-motile and non-spore forming. In old culture, the organism showed pleomorphic and long rod characteristics. During the investigation, various types of the colonies of the organism were also observed on blood agar. The organism appeared as non-haemolytic and exhibited moist, mucoid and shiny growth. It failed to grow on MacConkey’s agar. It was observed that vaccinal strain of Pasteurella multocida fermented glucose, Sucrose ,Maltose, Galactose , Mannose and Fructose but did not ferment lactose and salacin. It did not liquefy gelatin. Key Words: Pasteurella multocida, Vaccinal strain, Morphology, Biochemical characteristics.