Evaluation of Loop-Mediated Isothermal Amplification (LAMP) assay for diagnosis of Theileria annulata in both cattle and Buffaloes in Upper Egypt

Amira A. T. AL-Hosary1*, Laila S. Ahmed1 , Ulrike Seitzer2

1 Infectious Diseases, Department of Animal Medicine, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt, 2Veterinary Infection Biology and Immunology, Department of Cell Biology and Immunology, Research center-Borstel-Germany -Faculty of veterinary medicine, Assiut University, Assiut Egypt, Postal code 71526. - The work carried out in both faculty of veterinary medicine (department of animal medicine, infectious diseases), Assiut university, Assiut Egypt and Forschungszentrum- Borstel-Germany.

The corresponding author: Amira Adel AL-Hosary, (002)01004477501, faculty of veterinary medicine (department of animal medicine, infectious diseases), Assiut University, Egypt, fax (088)2366503, (088)2354130 E-mail, amiraelhosary@yahoo.com, d.miravet@yahoo.com

Abstract :

Loop-Mediated Isothermal Amplification (LAMP) assay was used for detection of Thaileria annulata infection in field samples from both cattle and buffaloes. These samples were collected from three governorates in Upper Egypt that include Assiut, EL-Fayoum and EL-wady EL-Gaded governorates. Reverse Line Blot (RLB) assay was used as a reference test for evaluation of LAMP assay efficacy in diagnosis of bovine theileriosis. The obtained results revealed that the infection rates according to the results of LAMP assay were (65.24% and 43.24%) in cattle and buffaloes, respectively. The evaluation study of LAMP test revealed high sensitivity in cattle if compared with buffaloes (78.10% and 47.37%), respectively. Specificity was higher in buffaloes if compared with cattle (61.11% and 57.53%), respectively. This study concluded that LAMP assay was sensitive and specific assay in diagnosis of Theileria annulata infection. So, it is recommended to use especially during molecular epidemiological surveys which should be applied on the wide range because it will give a clear picture about the epidemiology of the disease which helps in its prevention and control. Keywords: Diagnosis; LAMP; RLB; Theileria annulata.